Single particle analysis workflows.
Single particle analysis (SPA) is a group of related computerized image processing techniques used to analyze images from transmission electron microscopy (TEM). These methods were developed to improve and extend the information obtainable from TEM images of samples and allow for high resolution structure determination of proteins, protein complexes and viruses.
Cryo-EM single particle analysis: 300+ projects solved.
We are experienced in all stages of single particle analysis needs, from grid preparation and optimization, to high resolution data collection, through processing and refinement of the 3D map. We were the first, and continue to be the only, structural biology CRO with dedicated in-house Titan Krios microscopes, maintaining and expanding state-of-the-art facilities in both San Diego and the Greater Boston area.
We’ve collected data to support over 300 projects, solving over 150 structures, half of which were membrane proteins. Of the structures solved over 76% were determined at resolutions of 3.5 Å or better.
Types of Proteins We've Imaged
- Antibacterial Targets
- Antibody-antigen complexes
- DNA & RNA editing enzymes
- DNA Binding Proteins
- DNA Polymerases & DNA repair
- POLQ
- BRCA
- GPCRs
- Icosahedral (Adenovirus, AAV)
- Inflammasome
- Ion channels
- Macromolecular Complexes
- Ribosome
- Splicosome
- BAF
- Membrane proteins
- Multi-component complexes
- PROTACs
- Molecular Glues
- Protein degraders
- Protein-nucleic acid complexes
- Proteins - with and without ligands
- Solute Carrier Proteins (SLCs)
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